DEPURATION KINETICS AND BIOAUGUMENTATION STUDIES ON AMMONIA USING PSEUDOMONAS PUTIDA FOR AQUACULTURE SYSTEMSHEERTHANA V.R. AND PREETHA R.
The present depuration study explain bio cleansing activity of ammonia at lab level using immobilized P. putida cells with bentonite clay. The bentonite clay has wide scope for water treatment with healing properties. The bioaugumentation study was conducted to analyze the biodegradation ability of P.putida cells immobilized in bentoniteclay. The immobilization of the cells was carried out by adhesion technique using bentonite clay. The bentonite clay was separated from quartz and barium using elutriation technique using stokes law and other minerals present was characterized using scanning electric microscopy (SEM) and energy dispersive spectroscopy (EDS). The mineral studies conducted by elutriation technique using EDS shows the availability of molecular oxygen as 114.44% and carbon (17.58%) which ensured the microhabitat for the growth of P. putida cells. The study determined the effect of surface characteristics on the growth of bacteria depending on complex integrity between the physical, chemical and biological factors in the clay. Other micronutrients availability such as Fe (0.42%), Al (0.58%), and Si (0.84%) render the growth of bacteria and titanium present (0.11%) per weight helps in flocculation of other contaminants present in aquaculture systems. Advanced water treatment with bio filtration system facilitates aquaculture bioremediation to prevent quality and safety of fish and shellfish.
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