Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 1, 2018; Page No.(166-172)

CHARACTER OF LIQUID SEMEN MOTILITY IN VARIOUS DILUENTS ON BALINESE CATTLE DURING COLD STORAGE

TRINIL SUSILAWATI, DIAN RATNAWATI, NURUL ISNAINI, KUSWATI AND AULIA PUSPITAANUGRA YEKTI

Abstract

Balinese cattle have been spread and domesticated in Indonesia especially in Bali Island. Artificial insemination using liquid semen is a solution for applicated in areas where there is no liquid Nitrogen. The objective of this study was to observe the motility of Balinese Cattle spermatozoa by using liquid semen in Tris Aminomethane, CEP-2 and skim milk diluents. The materials used were two Balinese cattle. The research was located on Beef Cattle Research Station of Grati-Pasuruan. Semen was collected by using artificial vagina then continued by fresh semen analysis and liquid semen processing. Liquid semen quality was observed up to day 5 after chilled. The parameters measured were: sperm motility, sperm progressive motility, VCL, VSL, VAP, LIN, STR, WOB, H, ALH and BCF. Data were analyzed by minitab 17 and SPSS 16. The results showed that during cold storage, the sperm motility of Balinese cattle which were diluted with Tris aminomethane and CEP egg yolk was better than skim milk. Velocity linear curve (VCL), velocity average pathway (VAP), straightness (STR), and wobble (WOB) were decreased during cold storage. CEP-2 egg yolk can maintain the sperm motility better that other diluent.

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