PROXIMATE ANALYSIS, ANTIBACTERIAL ACTIVITY, TOTAL PHENOLIC CONTENT AND ANTIOXIDANT CAPACITY OF A GREEN MICROALGA SCENEDESMUS QUADRICAUDA (TURPIN) BRÉBISSONELDRIN DLR. ARGUELLES*
Methanolic extract of the algal strain Scenedesmus quadricauda (Turpin) BrĂ©bisson was subjected to microtiter plate dilution assay against a wide spectrum of bacteria. S. quadricauda exhibited antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa each having minimum inhibitory concentration (MIC) of 250 μg/mL. Listeria monocytogenes and Methicillin-resistant S. aureus were also moderately inhibited each having MIC of 1000 μg/mL. Minimum bactericidal activity (MBC) of S. aureus is higher than that of P. aeruginosa, having 500 μg/mL and 1000 μg/mL, respectively. On the other hand, L. monocytogenes, and Methicillin-resistant S. aureus exhibited MBC of >1000 μg/mL. The concentration of total phenolics of the methanolic extract determined by the folin-ciocalteu method was found to be 606.91 μg GAE/mL. DPPH free radical scavenging activity assay and CUPRAC assay were used to evaluate the antioxidant activity of the extract. Relative antioxidant efficiency showed that S. quadricauda exerted high ability of reducing copper ions from Cu (II) to Cu (I) and potent radical scavenging activity in a concentration dependent manner. The results further showed that the antioxidant activity of the extract was dose-dependent and positively correlated to their phenolic content. Proximate analysis of the dried microalgal biomass showed that S. quadricauda contains high protein content of 34.69+0.41%. Hence, S. quadricauda is a suitable candidate feedstock for the production of new bioactive compounds while its protein rich biomass can be used for animal feed.
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