OPTIMIZATION OF POLYHYDROXYALKANOATE (PHA) SYNTHESIS FROM BACILLUS SUBTILIS RS1R. RATHIKA, SOUMIYA MITHRAN, U. SARASWATHI, S. MANONMANI, S. KAMALA KANNAN AND K. SHANTHIAbstract Polyhydroxyalkanoate (PHA) is a complex class of storage polymer, synthesized by various bacteria and deposited as water insoluble intracellular organic inclusions. An experimental design was used to optimize the composition of the culture medium for enhanced production of PHA from Bacillus subtilis RS1. Sudan black B staining was used to observe the presence of PHA in B. subtilis RS1. Experimental variables selected for the optimization studies include type of media, pH, inoculum dosage, and incubation time. Several carbon and nitrogen sources were also assessed as media supplements. The amount of PHA accumulated in the cells followed a similar pattern to its growth in each treatment indicating growth-related production. The optimum conditions for maximum PHA production (~77%) were Luria Bertani broth amended with sucrose (2%), ammonium sulphate (1 g/L ), C/N ratio (20:1), inoculum dose 10% (v/v), pH 7.0 and incubation time of 48 h. The results of the study indicate that the isolate B. subtilis RS1 could be used for large-scale PHA production.
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