Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Feb. Suppl. Issue, 2018; Page No.(159-162)

CHLOROPHYLLID α BINDING PROTEIN GENE EXPRESSION FROM THE MIDGUT OF POLYVOLTINE KOLAR GOLD SILKWORM HYBRID

K.S MANJUNATHA GOWDA, ANITA PETER, N.B.N. MAHANTESHA, RAVINDRA GOUDA PATIL AND P. NIRANJANA

Abstract

Chlorophyllid α binding protein (chbp) was characterized by its ability to bind to the prosthetic group of chlorophylls and also involved in various physiological functions. Previous study on chbp have shown its high level of expression in the midgut of silkworm. But, no information is available on the influence of mulberry leaves fed to the silkworm insect and its effects on chbp expression. In the present study, polyvoltine Kolar Gold hybrid breed of PM x CSR2 was fed with V1 variety of mulberry green and yellow leaves separately, also a combination. Real-Time PCR analysis results showed that chpb was highly expressed in green leaves fed silk worms and declined in silkworm larvae¬ís fed with yellow leaves. Our results demonstrated that green leaves are responsible for the expression of chbp genes.

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