Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Feb. Suppl. Issue, 2018; Page No.(152-158)

INTRAGENERIC DIVERSITY OF TIMBER DEGRADING FUNGI FROM RATANMAHAL WILDLIFE SANCTUARY, GUJARAT, INDIA BY AFLP

PRAVEEN KUMAR NAGADESI, DUVVI NAVEEN BABU, K.S.M. PRASAD, PRAVEENA DEVI P., ARUN ARYA, P.D.V.N. SUDHEER AND SHAIK MASTAN

Abstract

Several molecular methods for detecting timber degrading fungi have been developed, but methods for direct extraction of fungal DNA and fungal species identification during the incipient stage of decay could benefit to save the timber from decay. Timber degrading fungi was collected from Rathanmahal Wildlife Sanctuary, Gujarat and brought to the lab for their DNA isolation, quantification, amplification and identification. Amplified Fragment Length Polymorphism analysis was used to determine the % variability in the genomic profiles of Lenites sterioides, L. betulina, L. exima, Flavodon flavus strain 1, F. flavus strain 2, Ganoderma lucidum, G. applanatum, Phelinus robustus, Schizophyllum commune, Pluerotus pulmonarius, and Stereum hirsutum. Using 4 combinations of AFLP selective primers 590 markers were generated out of which 586 makers were found to be polymorphic. On an average each primer has produced 49.16 markers with 48.88 polymorphic markers. The GS was also highest in case of AFLP as observed in between S. commune and Pleurotus sp. and minimum in between F. flavus strain 2 and G. lucidum. For the first time AFLP identification of Timber degrading fungi from Ratanmahal Wildlife Sanctuary was done. Forth first time different genera of Aphyllophorales isolates were compared for intrageneric diversity.

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