Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Feb. Suppl. Issue, 2018; Page No.(136-139)

STUDY OF ANTIBACTERIAL PROPERTIES OF LEAF EXTRACT OF NEEM (AZADIRCHTA INDICA) AGAINST SKIN BACTERIA

S. PANDA, M. MAHAPATRA, S. PANIGRAHI , S.P. NAYAK AND B.K. MOHANTY

Abstract

A study was undertaken to compare the antiseptic efficacy of two commercial products with active compounds like Chlorohexidine gluconate and chloroxylenol with crude leaf extracts of neem. Inoculums collected from the sample area of skin (forearm of hand) and cultured with plating techniques with varied treatments of commercial products and leaf extracts of neem. The results indicated that zone of inhibition decreased with the increase in the concentration of leaf extract and the methanol extract showed better inhibition zone than the aqueous extract of neem leaf. The MIC study indicated that the methanol extract showed effective inhibition at lowest concentration. The skin bacterial growth in different ways showed that least number of bacterial colonies were developed when grown with neem leaf extracts with methanol. Overall the study showed that the crude methanol extract of neem leaf was very effective against skin bacteria under experimental conditions.

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