Ecology, Environment and Conservation Paper

APPLICATION OF DNA MARKER (RAPD) TECHNOLOGY TO STUDY MOLECULAR DIVERSITY IN ADENIUM OBESUM (FORSSK), ROEM AND SCHULT

S.K. Chavan, Singh Alka and S.R. Barkule

Abstract

Broad genetic diversity of Adenium obesum is the key for genetic improvement of this important ornamental species.Use of seventeen arbitrary ten decamer oligonucleotide primers of four series viz. OPA, OPI, OPJ, OPN generated total 700 scorable bands with 155 loci, among them 148 loci were found polymorphic, showing 95% polymorphism. On an average, per primer 9 polymorphic loci were generated. The RAPD primers tested in present investigation produced fragments of different sizes. The minimum (121 Base pair) sized fragment was amplified by primer OPN-7, whereas maximum (2547 base pair) sized fragment was amplified by the same primer i.e. OPN-7. A highest (100%) polymorphism was exhibited by primers OPA- 1, OPA-9, OPJ-13, OPJ-17, OPI-19, OPI-20, OPJ-5, OPN-4, OPN-5, OPN-6, and OPN-7 while the lowest polymorphism percentage was recorded by two primer, i.e. OPA-18 and OPJ-121 with polymorphic value value of 66.66 per cent. Thus entire study revealed that genotype ‘Deang Udam Sap’ and ‘Picotte’ were found to be more genetically divergent genotypes followed by genotypes ‘Deang Udam Sap’ and ‘Mor Lok Dok’. Genotypes ‘Double Sweet heart’ and ‘Vithoon’s White’ were found to be genetically closest genotypes flowed by genotypes ‘Mor Lok Dok’ and ‘Picotte’. Genetic similarity between genotype ‘Arrogant’ and ‘Mung Siam Purple’ was also found to be considerable.