Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 4, 2017; Page No.(897-904)

IDENTIFICATION OF THE LEVULINATE DEHYDRATASE (ALAD) GENE POLYMORPHISM AND WHOLE BLOOD HEMOGLOBIN IN THE STUDENTS OF ELEMENTARY SCHOOL IN KALIDERES, JAKARTA, INDONESIA

RINI PUSPITANINGRUM, CHRIS ADHIYANTO, ANNISA FIRDAUSI, NURMASARI SARTONO1, RIA AMELIA, MELLA FERANIA, YUKIO HATTORI, YASUHIRO YAMASHIRO, RIANA BAGASKOROWATI AND SAPARUDDIN MUKTAR

Abstract

Lead (Pb) is a toxic pollutant and no organism can decrease the natural concentration. In Kalideres, there is a bus station and industries that are adjacent to settlements, exposing children around to Pb. One of the characteristics of Pb particulates is that they are found in the bottom layer of air, ± 1 meter above ground, equivalent to the height of children aged 6 to 9 years old (grade 1 to 2 elementary school students) whose height range between 100-140 cm, increasing the risk of the children being exposed to Pb. The aim of this study was to identify the ALAD gene polymorphism, to discover the frequency of the ALAD gene polymorphism genotype, and to discover the relationship between the ALAD gene polymorphism genotype and the hemoglobin concentration. The method used in this study was a comparative-descriptive method with laboratory test techniques using a digital hemoglobin meter, PCR and PCR-ARMS (Amplification Refractory Mutation System), and the completion of questionnaires by the parents for respondent behavioral characteristics androutines. The samples were 60 students who were 51.7% were ALAD wild types, 48.3% heterozygotic genotypes, and nomutants were found in the population of elementary schoolchildren in Kalideres, Jakarta, and there was no connection between the ALAD gene polymorphism genotype and the hemoglobin concentration category (anemicandnon-anemic). The results of this research also predict profile level of lead environmental present as well.

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