Ecology, Environment and Conservation Paper

Vol 23, Sept. Suppl. Issue 2017; Page No.(151-158)

PLANTLET REGENERATION IN GERBERA JAMESONII FROM CULTURED LEAF DISCS

Deepa Bhatt, M.K. Tripathi, Vidhya Sankar, M. and R.P. Patel

Abstract

In the present study, leaf disc were used as explants sources and cultured on MS medium amended with different auxins and cytokinins in varying concentrations as sole as well as in diverse combinations. Induction medium containing 2.0-3.0 mgL-1 auxins (2,4-D or NAA) promoted higher degree of callus induction. Nutrient medium supplemented with 2.0-3.0 mgL-1 NAA proved well for formation of morphogenic calli and regeneration medium MSB proved remarkably superior for plantlet regeneration in higher frequency. Higher in vitro rooting response (root proliferating efficiency, number of roots and mean root length) was exhibited by rooting medium MS.1IB (MS + 0.1 mgL-1 IBA + 15.0gl-1 sucrose + 7.5 g l-1 agar). The plantlets were transferred to pots and hardened in Environmental Growth Cabinet and Net House during preliminary wearing period and transferred to field successfully. Phenotypic normal plants were recovered.

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