STUDY BIOREMEDIATION POTENTIAL OF ISOLATED PSEUDOMONAS BACTERIES FROM KHARK ISLAND OIL SAMPLESAtousa Boozari1, Nooshin Sadjadi and Mojgan Emtyazjoo
Kharg Island because of its strategic location is one of the main centers of oil production and transport in the country that has been prone to severe oil pollution. Oil pollution is one of the most important environmental pollutions that cause damage to marine ecosystems and the creatures that depend on it. The objective of this study was to identify the deposits of crude oil degrading bacteria. Kharg Island is located in Bushehr. Oil-contaminated sediment samples collected and then placed in special containers were transported to the laboratory. In this study 11 strains were selected. Strains were capable of higher oil removed using specific culture, screening and were identified by biochemical and molecular methods. Results showed that oil degrading bacteria are Pseudomonas aeruginosa, Pseudomonas alcaligenes, Pseudomonas salomonii, Bacillus licheniformis, Acinetobacter calcoaceticus and their oil analysis were, respectively, 68/45, 65/72, 60/43, 58 / 25, 56/52 percent. The surface tension between different strains were between 28/5 to 49/3 mN / m, respectively. Given that there is a negative correlation between the ability to produce biosurfactant and decompose oil, it can be said that the ability to lower the surface tension of the samples is their ability to remove oil above the genus. Therefore, Pseudomonas with the most powerful disruptors has the lowest surface tension. So it can be concluded that the genus Pseudomonas, Bacillus and Acinetobacter are the most important crude oil degrading bacteria and the proliferation of the bacteria in field scale can be used to clean oil-polluted beaches.
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