Pollution Research Paper

Vol. 36, Issue 2, 2017; Page No.(197-204 )

OPTIMIZING CONDITIONS FOR CRUDE OIL DEGRADING BACTERIAL CONSORTIUM ISOLATED FROM AQUATIC ENVIRONMENT

M. AZAB EL-LIETHY, BAHAA A. HEMDAN, FARAG A. SAMHAN, SAHAR S. ALI AND GAMILA E. EL-TAWEEL

Abstract

Isolation and identification of fresh water light crude oil degrading bacteria was the aim of this in vitro study. Moreover, determining the optimum conditions for oil degrading activity. Total bacterial count and bacterial indicators were counted in parallel with oil degrading bacteria in sediment and water samples. Temperature, pH, COD and oil concentration were determined as the optimum factors for the activity of the oil degraders. The ability of oil degrading was tested via quantifying oil residues using gas chromatographs. The analysis of sediment samples showed the presence of some polycyclic aromatic hydrocarbons. After four frequent screenings, oil degrading bacteria were identified out of 225 isolates. Isolates has given the most potent crude oil degrading activity were identified as Pseudomonas aeruginosa, Coronobacter sakazakii, Klebsiella oxytoca and Bordetella bronchispetica consequently. Oil degraders were mesothermic bacteria with optimum pH activity from 7 to 7.5. Results can be concluded that, the consortium of the four bacterial strains showed high efficiency of oil degradation.

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