Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 1, 2017; Page No.(146-152)

BIODEGRADATION OF ORGANOPHOSPHOROUS PESTICIDE, CHLORPYRIFOS BY SOIL BACTERIUM - BACILLUS MEGATERIUM RC 88

M.A. CHANDRASHEKAR, M. SUPREETH, K. SOUMYA PAI, S.K.C. RAMESH N. GEETHA, H.R. PUTTARAJU AND N.S. RAJU

Abstract

Chlorpyrifos (CP), an Organ phosphorous pesticide is used around the world to control pest insects in agricultural sector. CP is registered for use in nearly 100 countries and applies to approximately 8.5 million crop acres each year. The crops with the most intense CP use are cabbage, cotton, cucumber, and other vegetable crops. A bacterial strain RC 88 capable of degrading CP was isolated from soil collected from agricultural field of Nanjangud Taluk of Mysore district, Karnataka, India by enrichment method. It was observed that, the strain RC 88 was able to utilize the CP as a sole carbon and energy source grown in Mineral salt medium containing 100mgL-1 of CP at 370C at 150 rpm in a rotary shaking incubator for about 7-14 days. The culture was extracted for CP at a frequency of the 7th and 14th day of incubation and was examined by LCMS. The strain RC 88 showed the ability of CP degradation by Hydrolyzing it into 3,5,6- trichloro-2-pyridinol (TCP). On the basis of morphology, physiological characterization and phylogenetic analysis of 16S rRNA sequence, the isolate was identified as Bacillus megaterium. The result obtained suggests that, the Bacillus megaterium sp strain RC 88 will be potentially useful in abatement of Chlorpyrifos contaminated soil.

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