MULTIVARIATE ANALYSIS OF ANTIOXIDANT ENZYMES AND PIGMENTS IN THE LEAVES OF PLANTS IN VICINITY OF RIVER BEAS, INDIAVinod Kumar, Anket Sharma, Renu Bhardwaj and Ashwani Kumar Thukral
Leaves of twenty one plant species were collected from the catchment area of river Beas, India and analyzed for antioxidant enzyme activities and pigments. SOD activity was found to be highest as compared to other antioxidant enzymes. Total chlorophyll content was found to be higher than carotenoids and anthocyanins. The first three components of principal component analysis (PCA) explained 86.94% and 99.98% of the total variance for enzyme activities and pigments respectively. Factor analysis for enzyme activities revealed that factor-1 had maximum positive loadings on detoxifying enzymes such as catalase (CAT) and superoxide dismutase (SOD). Factor-2 had maximum negative loadings on reductases such as dehydroascorbate reductase (DHAR) and glutathione reductase (GR). Factor-3 had maximum negative loadings on guaiacol peroxidase (POD) and ascorbate peroxidase (APX). This factor accounts the peroxidase enzymes. Factor analysis of pigments showed factor-1 contributing to photosynthetic pigments such as chlorophyll and carotenoids. Factor-2 had maximum loadings on anthocyanins. Multiple linear regression analysis showed that CAT had maximum positive regression on the SOD activity, whereas POD negatively regressed on it. Further MLR also revealed that chlorophyll content is positively regressed on carotenoid content.
Enter your contact information below to receive full paper.