Ecology, Environment and Conservation Paper

VARIETY OF APPLICATIONS OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) IN PLANTS

Chandra Kant Sharma, Snehal Kanole, Monika Sharma1 and Vinay Sharma

Abstract

HPLC (High Performance Liquid Chromatography) is the combination of Liquid Chromatography & Gas Chromatography which provides easy separation of the any molecule, high resolving power, HPLC has an essential place in the field of analysis of phenolic compounds. It is the most sensitive & selective techniques for the plant extract analysis. Currently, HPLC is the necessity in food industries, thermal paper industries, pharmaceutical industries and healthcare etc. It is possible to identify and determine very low amounts of analytes in the presence of mixture of various components like carotenoids and chlorophyll, quercetin, natural dyes, pesticides, vegetable oils etc. Various types of plant samples such as apricot, caffeine, apple, peach, aloe vera, walnut, henna etc. can be used in HPLC in different industries. HPLC is the accurate, stable and most convenient techniques used for determination of secondary metabolites, flavonoids and sterols present in plants for food & medicinal applications. Combination of HPLC & MS techniques allows identification as well as quantitative analysis of phenolic compounds even in very low concentrations.