Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.12, Issue 3, 2010; Page No.(663-665)

AMINO ACIDS COMPOSITION IN NATURAL AND IN VITRO GROWN PLANTS OF GLOSSOCARDIA BOSVALLEA L.F(DC)

R. GEETHA AND G.V. GOPAL

Abstract

Detection of amino acids in natural and tissue culturally raised plants of Glossocardia bosvallea was made using two dimensional paper chromatographic technique using Butanol: Acetic acid: Water (9:1:1) and Phenol: Water (3:1) as solvent system I and II respectively. Significant variations in the composition of amino acids of both in vitro and in vivo grown plants were recorded. The in vitro plants were established in MS media supplemented with 2 mg/L BAP. Naturally grown plants were found to contain greater number of amino acids when compared to in vitro grown plants. Absence of DL aspartic acid, DL alanine, DL-B-Phenyl alanine, DL valine were noticed in in vitro grown plants which have made their marked presence in naturally grown ones.

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