MULTIVARIATE ANALYSIS REVEALS THE ROLE OF 24- EPIBRASSINOLIDE IN ELEMENTAL UPTAKE BY BRASSICA JUNCEA L. UNDER IMIDACLOPRID TOXICITYANKET SHARMA, VINOD KUMAR, MUKESH KUMAR KANWAR, ASHWANI KUMAR THUKRAL AND RENU BHARDWAJAbstract An experiment was designed to observe the effects of 24-epibrassinolide (EBR) seed-soaking on elemental composition of the leaves of Brassica juncea plants grown in soils amended with imidacloprid (IMI) pesticide. Scanning electron microscope-energy dispersive X-ray spectrometer (SEM-EDX) analysis of the leaves of plants showed that carbon, nitrogen, sodium, magnesium, phosphorous, sulphur, potassium and calcium contents were decreased when the plants were grown in IMI amended soils. However, seed treatment with EBR enhanced the elemental composition of plants grown under IMI toxicity. Multivariate analysis of the data was done using two-way analysis of variance followed by Tukeys multiple comparison test, multiple linear regression, β-regression coefficients, principal component analysis and artificial neural networks. It was concluded that pre-sowing seed treatment with EBR enhanced the elemental contents in B. juncea plants grown under IMI toxicity.
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