ALKALINE DELIGNIFICATION AND BIOETHANOL
PRODUCTION FROM AGRO-RESIDUESRAGHAVENDRA B. HAVANNAVAR AND G. S. GEETA
Abstract
Bioethanol is produced by fermentation of sugarcane molasses using strains of Saccharomyces
cereviseae. But recent increase in cost of molasses, it has been emphasized to search alternate substrates for
ethanol production. The agroresidues such as paddy straw, wheat straw and sugarcane bagasse as an
alternate source for ethanol production were tested. In the present study, pretreatment process was
developed for hydrolyzing paddy straw, wheat straw and sugarcane bagasse into fermentable sugars. The
powdered substrates were pretreated with 2 and 3 per cent sodium hydroxide (NaOH) for different
incubation periods. The substrate pretreated with 3 per cent NaOH for 8 h has given maximum cellulose
content in sugarcane bagasse 0.695 g/g followed by wheat substrates were subjected for commercial
cellulase enzyme pretreatment for further saccharification. The highest reducing sugar was recorded in
sugarcane bagasse (136.86 mg/g), which was found significantly superior over other substrates. The
hydrolysate thus obtained was used for screening of yeast cultures. Among yeast strains Pouhysolen
tannophilus NCIM 3445 has showed comparatively higher ethanol yield in all the substrates.
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