Ecology, Environment and Conservation Paper

Vol 22, Sept. Suppl. Issue , 2016; Page No.(423-428)

MOLECULAR CHARACTERIZATION OF CHICKPEA BACTERIAL BIOAGENTS AGAINST DRY ROOT ROT BY RAPD AND 16S RDNA TECHNIQUES

G. Amrutha Veena, N.P. Eswara Reddy, B.V. Bhaskara Reddy3 and L. Prasanthi

Abstract

RAPD banding profile with four different random primers viz., OPA-12, OPA-18, OPD-4 and OPD-5 revealed the existence of genetic variability among the eight isolates that were classified into 3 main clusters. Cluster I is sub-divided into cluster Ia (CREB-13 and CREB-6) and Ib (CRB-20). cluster-II contains CREB-16 and CRB-6. Cluster III is sub-divided into cluster Ic (CRB-12 and CRB-9) and Id (CREB-10). Amplification of 16S rDNA with 63F and 1387R primers which are specific to bacterial 16S rDNA produced approximately 1300 bp fragment. These results show that the potential antagonistic isolate CREB-16 was Bacillus subtilis that belong to prokaryotes.

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