Ecology, Environment and Conservation Paper

Vol 22, Sept. Suppl. Issue , 2016; Page No.(363-366)

SCREENING OF RHIZOBIAL AND PSEUDOMONAS FLUORESCENCE ISOLATES FOR ABIOTIC STRESS TOLERANCE

K. Manasa, R. Subhash Reddy, S. Triveni, B. Kranthi Kumar

Abstract

An attempt has been made to evaluate the effect of abiotic constraints (salt, pH, drought and temperature) on the growth of Rhizobial and Pseudomonas fluorescence isolates. These 30 bacterial isolates were isolated from the different rhizosphere soils of Groundnut, Black gram, Green gram, Red gram, Soybean, Sunflower, Maize and Rice soils of student farm and college farm, College of Agriculture, Rajendranagar, PJTSAU, Hyderabad, Telangana statewith a view to screen out stress tolerant isolates. A total of 30 isolates have been used for screening their stress tolerating ability with contrast to environmental abiotic soil conditions. All the isolates were phenotypically and biochemically characterized followed by experiment under controlled environmental conditions.On the basis of comparison of growth under varied stress conditions, The PGPR with multiple beneficial activity with additional capabilities to be tolerant to abiotic stresses such as temperature, drought, pH, salinity and heavy metals such as As, Cd & Hg would help in identifying two Rhizobial isolates RR-1, GNR-1 and two Pseudomonas fluorescence isolates GGP-1, MP-1. These PGPR are eco-friendlyin nature and cost effective and therefore their use in crop production makes agriculture profitable and sustainable.

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