Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.12, Issue 3, 2010; Page No.(489-494)

SCREENING OF MEDIUM COMPONENTS FOR PRODUCTION OF THERMOSTABLE AMYLOPULLULANASE BY CLOSTRIDIUM THERMOSULFUROGENES SVM17 IN SUBMERGED FERMENTATION USING PLACKETT-BURMAN DESIGN

S. MRUDULA1*, GOPAL REDDY2 AND G. SEENAYYA3

Abstract

xScreening of carbon, nitrogen, trace minerals and complex organic sources on production of thermostable amylopullulanase by Clostridium thermosulfurogenes SVM17 in submerged fermentation (SmF) was carried out by employing Plackett-Burman design, containing a total of fifteen medium components in sixteen experiments. The design involves screening of up to n-1 nutrients in just n number of experiments. Regression coefficients and t-values were obtained by subjecting the results to statistical analysis. Among the fifteen medium components screened, except calcium chloride and tryptone, remaining all other nutrients showed significant effects on both amylase and pullulanase production. Thus, soluble starch, tapioca flour, peptone, magnesium chloride and ferrous sulfate were considered promising sources for production of the enzyme.

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