OPTIMIZATION OF MEDIA BY RESPONSE SURFACE METHODOLOGY FOR THE REDUCTION OF CHROMIUM BY BACILLUS SP.RENGANATHAN KASIMANI1, RENGANATHAN SEENIVASAGAN, SADASIVAM MUNRAJI, CHELLAPPAN BALAGRURNATHAN1 AND KRISHNAN SUNDAR
Sixteen chromium-resistant bacterial strains were isolated from tannery effluent collected from Dindigul and Walajabadh in the state of Tamilnadu, India. These isolates displayed different degrees of chromate reduction under aerobic conditions. One of the 16 isolates, which was resistant to 500 mg/L of potassium dichromate (Cr(VI)) was identified as Bacillus sp. The effectiveness of the bacterium in reducing Cr(VI) under different conditions were evaluated. Influences of various parameters like carbon (0.5 - 2.5 mg/ L), nitrogen (0.5 -2.5 mg/L), pH (5-9) and, time on chromium (VI) reduction were examined using response surface methodology. The interactions between the parameters were studied using Central Composite Design. The maximum percentage reduction of chromium (VI) was found to be 92.98%.
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