Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.12, Issue 4, 2010; Page No.(857-861)

HISTOCHEMICAL ASSESSMENT OF MUCOSUBSTANCES IN PANCREAS OF FLUORIDATED RATS

SHASHI A., N. SHARMA, M. BHARDWAJ AND S. NEETIKA

Abstract

The present study was undertaken to find out cytochemical changes in mucosubstances in pancreas. Sixty Spargue Dawley albino rats were injected with 30, 45 and 75 mg NaF/kg bw/days for 36 days. The rats of control group were administered subcutaneously double distilled water 1cc/kg bw/day daily for the same period. The experimental period was divided into 4 phases at interval of 15, 22, 30 and 36 days. Animals were sacrificed from each group at 16, 23, 31, and 37 days. There was intense distribution of PAS positive mucosubstances in the acini as well as in the Islets of Langerhans in the pancreas of control rat. The nuclear and cell membrane of exocrine and endocrine cells stained positive for periodic schiff's stain. The mucosubstances in all cellular components of pancreas were highly elevated in all fluoridated groups of rats as compared to control.

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