Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.12, Issue 4, 2010; Page No.(925-925)

EFFECT OF DIFFERENT NITROGEN SOURCE AND BARIUM SULPHATE ON ASPERGILLUS CLAVATUS. DESM

GANESH IYER AND SEEMA MENON

Abstract

Aspergillus clavatus Desm. Was isolated from paddy soil of thane district and pure culture was grown and maintained in czapeks agar. Aspergillus clavatus Desm. Is characterized by large vesicle which is elongated and fertile over an area of 200-250 microns. The mycelial mat was white to pale blue in colour (Subramanian 1971). Aspergillus clavatus is industrially less utilized and has potential to produce anti microbial drug (James et al. 2010) and alkaline protease.To understand the growth requirement, the innoculum was made on potato dextrose agar and different nitrogen source like peptone, ammonium chloride and ammonium sulphate of different concentration was used. Plates were incubated for 5 days at 37 C . Biomass of mycelium was determined by overnight drying at 85 C. Peptone with a concentration of 1% showed higher mycelial weight. Similarly in potato dextrose agar along with 1% peptone different concentration of BaCl2 ( 10 mg ,50 mg, and 100mg) were added and incubated at 37C for 5 days. Mycelial dry biomass was determined , which showed higher in 10 mg of BaCl2 and mycelial mat was white in colour where as in 100 mg of BaCl2 the mycelium was blue in colour. This gives an idea that the mycelial colour can be enhanced and useful blue colour dye can be obtained.Aspergillus clavatus Desm. Was isolated from paddy soil of thane district and pure culture was grown and maintained in czapeks agar. Aspergillus clavatus Desm. Is characterized by large vesicle which is elongated and fertile over an area of 200-250 microns. The mycelial mat was white to pale blue in colour (Subramanian 1971). Aspergillus clavatus is industrially less utilized and has potential to produce anti microbial drug (James et al. 2010) and alkaline protease.To understand the growth requirement, the innoculum was made on potato dextrose agar and different nitrogen source like peptone, ammonium chloride and ammonium sulphate of different concentration was used. Plates were incubated for 5 days at 37 C . Biomass of mycelium was determined by overnight drying at 85 C. Peptone with a concentration of 1% showed higher mycelial weight. Similarly in potato dextrose agar along with 1% peptone different concentration of BaCl2 ( 10 mg ,50 mg, and 100mg) were added and incubated at 37C for 5 days. Mycelial dry biomass was determined , which showed higher in 10 mg of BaCl2 and mycelial mat was white in colour where as in 100 mg of BaCl2 the mycelium was blue in colour. This gives an idea that the mycelial colour can be enhanced and useful blue colour dye can be obtained.Aspergillus clavatus Desm. Was isolated from paddy soil of thane district and pure culture was grown and maintained in czapeks agar. Aspergillus clavatus Desm. Is characterized by large vesicle which is elongated and fertile over an area of 200-250 microns. The mycelial mat was white to pale blue in colour (Subramanian 1971). Aspergillus clavatus is industrially less utilized and has potential to produce anti microbial drug (James et al. 2010) and alkaline protease.To understand the growth requirement, the innoculum was made on potato dextrose agar and different nitrogen source like peptone, ammonium chloride and ammonium sulphate of different concentration was used. Plates were incubated for 5 days at 37 C . Biomass of mycelium was determined by overnight drying at 85 C. Peptone with a concentration of 1% showed higher mycelial weight. Similarly in potato dextrose agar along with 1% peptone different concentration of BaCl2 ( 10 mg ,50 mg, and 100mg) were added and incubated at 37C for 5 days. Mycelial dry biomass was determined , which showed higher in 10 mg of BaCl2 and mycelial mat was white in colour where as in 100 mg of BaCl2 the mycelium was blue in colour. This gives an idea that the mycelial colour can be enhanced and useful blue colour dye can be obtained.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!