Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.12, Issue 4, 2010; Page No.(925-925)

EFFECT OF DIFFERENT NITROGEN SOURCE AND BARIUM SULPHATE ON ASPERGILLUS CLAVATUS. DESM

GANESH IYER AND SEEMA MENON

Abstract

Aspergillus clavatus Desm. Was isolated from paddy soil of thane district and pure culture was grown and maintained in czapeks agar. Aspergillus clavatus Desm. Is characterized by large vesicle which is elongated and fertile over an area of 200-250 microns. The mycelial mat was white to pale blue in colour (Subramanian 1971). Aspergillus clavatus is industrially less utilized and has potential to produce anti microbial drug (James et al. 2010) and alkaline protease.To understand the growth requirement, the innoculum was made on potato dextrose agar and different nitrogen source like peptone, ammonium chloride and ammonium sulphate of different concentration was used. Plates were incubated for 5 days at 37 C . Biomass of mycelium was determined by overnight drying at 85 C. Peptone with a concentration of 1% showed higher mycelial weight. Similarly in potato dextrose agar along with 1% peptone different concentration of BaCl2 ( 10 mg ,50 mg, and 100mg) were added and incubated at 37C for 5 days. Mycelial dry biomass was determined , which showed higher in 10 mg of BaCl2 and mycelial mat was white in colour where as in 100 mg of BaCl2 the mycelium was blue in colour. This gives an idea that the mycelial colour can be enhanced and useful blue colour dye can be obtained.Aspergillus clavatus Desm. Was isolated from paddy soil of thane district and pure culture was grown and maintained in czapeks agar. Aspergillus clavatus Desm. Is characterized by large vesicle which is elongated and fertile over an area of 200-250 microns. The mycelial mat was white to pale blue in colour (Subramanian 1971). Aspergillus clavatus is industrially less utilized and has potential to produce anti microbial drug (James et al. 2010) and alkaline protease.To understand the growth requirement, the innoculum was made on potato dextrose agar and different nitrogen source like peptone, ammonium chloride and ammonium sulphate of different concentration was used. Plates were incubated for 5 days at 37 C . Biomass of mycelium was determined by overnight drying at 85 C. Peptone with a concentration of 1% showed higher mycelial weight. Similarly in potato dextrose agar along with 1% peptone different concentration of BaCl2 ( 10 mg ,50 mg, and 100mg) were added and incubated at 37C for 5 days. Mycelial dry biomass was determined , which showed higher in 10 mg of BaCl2 and mycelial mat was white in colour where as in 100 mg of BaCl2 the mycelium was blue in colour. This gives an idea that the mycelial colour can be enhanced and useful blue colour dye can be obtained.Aspergillus clavatus Desm. Was isolated from paddy soil of thane district and pure culture was grown and maintained in czapeks agar. Aspergillus clavatus Desm. Is characterized by large vesicle which is elongated and fertile over an area of 200-250 microns. The mycelial mat was white to pale blue in colour (Subramanian 1971). Aspergillus clavatus is industrially less utilized and has potential to produce anti microbial drug (James et al. 2010) and alkaline protease.To understand the growth requirement, the innoculum was made on potato dextrose agar and different nitrogen source like peptone, ammonium chloride and ammonium sulphate of different concentration was used. Plates were incubated for 5 days at 37 C . Biomass of mycelium was determined by overnight drying at 85 C. Peptone with a concentration of 1% showed higher mycelial weight. Similarly in potato dextrose agar along with 1% peptone different concentration of BaCl2 ( 10 mg ,50 mg, and 100mg) were added and incubated at 37C for 5 days. Mycelial dry biomass was determined , which showed higher in 10 mg of BaCl2 and mycelial mat was white in colour where as in 100 mg of BaCl2 the mycelium was blue in colour. This gives an idea that the mycelial colour can be enhanced and useful blue colour dye can be obtained.

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