ASSESSMENT OF GENETIC DIVERSITY IN CHERRY TOMATO (SOLANUM LYCOPERSICUM L. VAR. CERASIFORME) GENOTYPES USING SSR MARKERSKiran Kumar, J. Trivedi D. Sharma , Vikrant Sahu and G. Chandel
The present investigation regarding study of genetic variation of 15 genotypes including Pusa Ruby of cherry tomato (Solanum lycopersicum L. var. cerasiforme) started with the screening of polymorphic microsatellite markers. From the identified 10 polymorphic SSR markers, a total of 142 polymorphic amplified fragments were obtained. The average polymorphism information content (PIC) was 0.987 and ranging from 0.958 (SSR-47) to 0.996 (SLM6-18). One hundred fourty two SSR loci were used to calculate Jaccards distance coefficients for UPGMA cluster analysis. The generated dendrogram of 15 cherry tomato genotypes showed low level of similarity among these genotypes. Two major clusters were generated at 23% level of similarity level. The major cluster A consists of five genotypes whereas major cluster B consists of rest of 10 genotypes. The result also showed that, maximum, 89% of similarity was found between Cherry T.1×Co - 3-2 and Cherry T.4 × Pant T. -3 genotypes. The present study demonstrated that SSR markers are effective for obtaining unique fingerprints of, and assessing genetic diversity among, tomato wild relatives and would be used for wide range of practical application including varietal identification and pre-screening for distinctiveness of tomato genotypes.
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