Ecology, Environment and Conservation Paper

Vol. 22, June Suppl. Issue 2016; Page No.(137-142)

ASSESSING GENETIC DIVERSITY OF GLADIOLUS (GLADIOLUS HYBRIDUS L.) GENOTYPES USING SEQUENCE RELATED AMPLIFIED POLYMORPHISM (SRAP) MARKERS

R. Rashmi, D.C. Lakshmanreddy and S.Y. Chandrashekar

Abstract

Genetic diversity with in Gladiolus genotypes (Gladiolus hybridus L.) is the key to genetic improvement of this important ornamental species. In the present study, genetic diversity of 20 gladiolus genotypes were assessed through Sequence Related Amplified Polymorphism (SRAP) markers. Sequence Related Amplified Polymorphism (SRAP) is a new molecular marker technology developed based on PCR. SRAP analysis were carried out using twenty five primer combinations out of which, thirteen primer combinations revealed a total of 220 scorable bands, among which 176 were polymorphic with an average of 13.54 polymorphic bands per pair of primers. The percentage of polymorphism ranged from a maximum of 93.33 percent by Me 3 + Em 3 primer combination to a minimum of 50.00 percent by Me 2 + Em 6. The SRAP profiles generated were further evaluated for studying the JaccardÂ’s similarity coefficient. The similarity matrix coefficient ranged from 18 to 79 percent, suggesting a higher genetic variation within gladiolus genotypes. On comparing the genetic diversity as revealed by the dendrogram, it was evident that Shobha a mutant genotype was identified quite distinct from the other genotypes.

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