EVALUATION OF GENETIC DIVERSITY IN GROUNDNUT MUTANTS (ARACHIS HYPOGAEA L) USING SEED PROTEIN ELECTROPHORESISM. R. Kharade, V. V. Ujjainkar and K. C. Bhagat
Seed storage proteins of nine mutants of groundnut genotype (CTMG-6-1) were analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis with a view to assess genetic diversity. Based on the relative mobility of total soluble seed protein using SDS-PAGE, 80 bands were detected in this experiment, which were used for examining the diversity among mutants. In detected 80 bands, 51 major bands were found to be monomorphic whereas, remaining 29 bands were polymorphic in nature. The banding pattern revealed variation among the mutants whereas little poly morphism was detected within the mutants. Relative mobility value of protein on polyacrylamide gel (SDS-PAGE) for these mutants under study showed substantial variation for seed protein profiles among the mutants of groundnut. The high yielding mutants exhibited bands having Rm values ranging from 0.20 to 0.98 along with varying banding pattern within the group confirming the complex nature of the trait. With this study, it may be concluded that the SDS-PAGE is an effective tool for evaluating genetic diversity among the genotypes or mutants. Also electrophoretic pattern may be used for rapid screening of available material for the traits under investigation.
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