Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.12, Issue 4, 2010; Page No.(831-838)

ISOLATION AND CHARACTERIZATION OF P-SOLUBILIZING PSEUDOMONAS PUTIDA BIOTYPES IN TWO MICROBIOLOGICAL GROWTH MEDIA (PKVS AND NBRIP) FROM RHIZOSPHERE SOILS OF GROUNDNUT IN TIRUPATI

K. JYOTHI, K. VIJAYALAKSHMI , M.SUREKHARANI, S. ARUNA, M. SHASHIKANTH, N. D.PRASANNA AND S.K. SHAHEEN

Abstract

Pseudomonas putida biotypes (Ps1, Ps2, Ps3, Ps4) isolated from rhizosphere soils of ground nut plants growing in fields of Tirupati, A.P. Phosphate solubilizing activity of Psuedomonas putida biotypes was screened in solid and liquid formulations of PKVS ( Pikovskays medium) and NBRIP (National Botanical Research Institutes Phosphate growth Medium) media. Identification of Pseudomonas putida biotypes depending on morphological, biochemical tests, utilization of carbon and nitrogen sources, characterization according to Bergeys Manual of Systematic Bacteriology Vol.I. Efficient phosphate solubilizer (Ps1) was identified as Pseudomonas putida biotype A by FAME analysis (IMTECH, Chandigarh). NBRIP served as best medium for phosphate solubilization to Psuedomonas putida biotypes with maximum solubilization of inorganic phosphorus associated with drop in pH during 16th day of incubation with statistically significant results.

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