Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 2, 2016; Page No.(417-422)

ISOLATION, CHARACTERIZATION AND ANTIBIOTIC RESISTANCE PATTERN OF NOSOCOMIAL BACTERIAL ISOLATES

YASMIN AKTAR BANU, MAYUR SWAMY AND VIDYA TALE

Abstract

Nosocomial or Hospital acquired Infections (HAI) are a critical challenge to public health as these infections are one of the leading cause of death. Bacterial infections due to antibiotic resistant microorganisms are becoming increasingly common. This study was focused on analyzing the pattern of antibiotic resistance in nosocomial pathogens. Thirteen bacteria were isolated from a hospital in Pune cityand identified using standard morphological and biochemical tests. Pattern of antibiotic resistance was assessed with thirteen different antibiotics as per the guidelines of Clinical and Laboratory Standards Institute (CLSI). The results showed that among the thirteen isolates, Nisseria spp. (30.77%) was predominant followed by Pseudomonas spp. (15.38%), Moraxella spp. (15.38%), Hemophilus spp. (7.69%), Morococcus spp. (7.69%), Brucella spp. (7.69%), Bacillus spp. (7.69%) and Staphylococcus spp. (7.69%). Most of the isolateswere highly resistant to the different antibiotics used. Resistance frequency of Tetracycline was highest (80%) towards both Gram negative and Gram positive organisms followed by Azithromycin (67%). The increasing trend of resistance among the nosocomial pathogens poses a great health problem and in order to combat this, we need better and effective drugs to control and manage nosocomial infections.

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