Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 2, 2016; Page No.(315-327)

MOLECULAR DETECTION AND CLUSTER ANALYSIS OF VACUOLAR SERINE PROTEASE GENE IN PENICILLIUM ISOLATED FROM HOSPITAL INDOOR AIR IN YOGYAKARTA, INDONESIA

ANDIKA SIDAR, NOVA W. PRATIWI, JAKA WIDADA, LATIFFAH ZAKARIA AND ENDANG S. RAHAYU

Abstract

Penicillium are commonly found in indoor air and some of them are known as allergenic fungi. Vacuolar serine protease (VSP) was reported as major allergens of several Penicillium species. Existence of allergenic fungi is considered as a risk factor for inhabitants inside the room, especially in hospital. So far, no studies are available about hospital airborne fungi in Indonesia. Therefore, this study was conducted to investigate fungal isolates obtained in hospital indoor air and then focused on detection as well as cluster analysis of VSP allergenic Penicillium. All fungal genera obtained were identified in genus level based on morphological characteristics. Fungal group belong to Penicillium were then collected for further molecular detection based on Polymerized Chain Reaction (PCR) amplification of VSP gene using new degenerate primer design (VP1 and VP2) and continued with species identification based on β-tubulin gene. Cluster analysis was exhibited by phylogenetic relationship among VSP allergenic Penicillium isolates based on nucleotide sequence of VSP gene. The result showed that genus of Penicillium was found as dominant airborne fungi from several hospital rooms. Moreover, presence of VSP gene was detected in P.citrinum, P. steckii, and P. copticola. Interestingly, the existing of VSP gene in P. steckii and P. copticola is the first time reported especially in Indonesia. Furthermore, phylogenetic tree constructed as cluster analysis showed that VSP gene of the same Penicillium species was grouped in one cluster. It indicates that gene encoding VSP allergen might be classified as species-specific.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 21, Issue 4, 2019
Vol 21, Nov. Suppl. Issue, 2019
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!