STUDY OF SUITABLE CULTURE MEDIA AND OTHER ABIOTIC FACTORS FOR THE GROWTH AND SPORULATION OF MAGNAPORTHE ORYZAEVarsha Gayatonde, P. Mahadevu and Prudhvi Raj Vennela
In the present investigation the effect of media, photo-periods and isolation techniques influenced on mycelia growth and conidial production of M. oryzae were studied. Rice straw agar and oatmeal agar wre found to be suitable for culturing different isolates of M. oryzae. Colonies of M. oryzae appeared white on oat meal, whitish grey on rice agar and grey on potato dextrose agar. When spores of 26 isolates were compared, MTU 1010 and Karidoddi showed the maximum size of conidia whereas isolate Kempudoddi showed minimum size of conidia. Size of the conidia from the infected samples varied from 13.8-21.35 μm to 2.7-7.8 μm (L x B) in all isolates where there was no significant variation in shape of conidia. The spores of rice isolates collected from Ponnampet were smaller as compared to Mandya. Highest radial growth of mycelia on Sixteenth day was shown on three medias from three rice varietal isolates Viz, Kempudoddi (83.23 mm) on OMA, Gamnadabhatta (70.42 mm) PDA, Honasu (75.3 mm) on RSA.
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