Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

DECOLORIZATION OF DIFFERENT DYES BY ISOLATED BACTERIAL STRAINS

MANISHA SHARMA, NEETIKA MATHUR, VINOD KUMAR NIGAM* AND PURNENDU GHOSH

Abstract

Three different bacterial isolates were tested for their decolorization efficiency on a number of dyes in both agar plates and liquid culture conditions respectively. The growth and decolorization efficiency of the isolated cultures were also evaluated in presence of different carbon sources and it was found that there was no effect of carbon sources in the decolorization of methyl red but it plays a crucial role in the decolorization of other dyes (red RH, brown GR, orange 3R etc). Two of the isolates BISR D-01 and BISR D-02 were observed with maximum decolorization capacity in all the cases at optimal conditions. The decolorization was confirmed by studying the spectral analysis of the respective dye. The growth kinetics showed that methyl red was completely decolorized within 18 hours of incubation.