BIOSYNTHESIS AND CHARACTERIZATION OF SILVER NANO PARTICLES USING METSCHNIKOWIA PULCHERRIMA STRAIN 29A: THEIR ANTIBACTERIAL, ANTIFUNGAL AND BIOLUMINESCENT TOXICITY EFFECTS AGAINST MICROBIAL PATHOGENSAyman Kamal, Sahar Zaki, Gadallah Abu-Elreesh and Desouky Abd-El-Haleem
In this study, the yeast isolates 29A identified as Metschnikowia pulcherrima was employed as a biocatalyst to synthesize silver nano particles (AgNPs). AgNPs was characterized by UV-vis, XRD, EDX, SEM and TEM. Optimization of AgNPs production and their antimicrobial activity were studied. Data showed maximum absorbance of 420425nm at pH 7, 30°C, and 7mM AgNO3 with AgNPs size ranged from 2 to 18nm. AgNPs toxicity was monitored using the bioluminescent bioreporter DF4/PUTK2. It was sensitive to AgNPs from 1 to 100μL. All analysis supported the intracellular production of AgNPs. AgNPs were able to inhibit the growth of all examined microorganisms. SEM and TEM analysis showed many pits and gaps on the cell surface of bacteria and destruction of the membrane and mycelia of fungi. In closing, this is the first report of synthesis of AgNPs using Metschnikowia pulcherrima 29A and their significant antibacterial and fungicidal action.
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