Ecology, Environment and Conservation Paper

Vol. 22, April Suppl. Issue 2016; Page No.(317-325)

ASSESSMENT OF GENETIC VARIATION IN TOMATO (SOLANUM LYCOPERSICUM L.) GENOTYPES USING SSR MOLECULAR MARKERS

Diklesh Kumar , Neeraj Shukla, Vikrant Kumar Sahu Dhananjay Sharma1 and Girish Chandel

Abstract

The present investigation regarding study of genetic variation of 19 genotypes of tomato (Solanum lycopersicum L.) started with the screening of polymorphic microsatellite markers. From the identified 11 polymorphic SSR markers, a total of 261 polymorphic amplified fragments were obtained. The average polymorphism information content (PIC) was 0.990 ranging from 0.979 (SSR-110) to 0.995 (SSR-253). Two hundred sixty one SSR loci were used to calculate JaccardÂ’s distance coefficients for UPGMA cluster analysis. The generated dendrogram of 19 tomato genotypes showed low level of similarity among these genotypes. Two major clusters were generated at 43% level of similarity. The cluster A consists of two genotypes whereas cluster B consist of rest of 17 genotypes. The results also showeds that the genotypes Arka Vikas and 2012TODVAR-2 were 100% similar. Therefore, these markers may be used for wide range of practical application including varietal identification and pre-screening for distinctiveness of tomato genotypes.

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