Ecology, Environment and Conservation Paper

IDENTIFICATION OF PUTATIVE PARENTS FOR DEVELOPING POPULATIONS FOR MAPPING GENOMIC REGIONS CONTROLLING ECONOMICALLY IMPORTANT TRAITS IN DOLICHOS BEAN (LABLAB PURPUREUS L.) VAR. LIGNOSUS

C. M. Keerthi, S. Ramesh, M. Byregowda, P. V. Vaijayanthi1, N. Mangalagowri M. Showkath Babu and A. Mohan Rao

Abstract

Identification of suitable parents contrasting for target traits as well as for a set of large number of DNA markers is a prerequisite for developing mapping population for use in identification of DNA markers linked to genomic regions controlling target traits. The linked DNA markers would serve as powerful surrogates for selection of target traits to enhance pace and efficiency of breeding crop plants. An investigation was carried out during 2014-15 to identify putative parents contrasting for pod yield its component traits and a set of simple sequence repeat (SSR)-based markers for use indeveloping mapping populations in dolichos bean. The per se performance of16 advanced breeding genotypes was assessedfor four quantitative traits and genotyped using 55 SSR based markers at the experimental plot and plant molecular biology laboratory, respectively of the Department of Genetics and Plant Breeding, University of Agricultural Sciences (UAS), Bengaluru, India. Based on quantitative traitsper se performance, the pairs of genotypes such as RIL 21 and RIL 25 vs. HA 10-8, HA 11-3, HA 4, HA 3, FPB 15, FPB 8, FPB 3 and FPB 21 were identified as contrasting for all the four traits. From among these,three pairs of contrasting genotypesnamely, HA 3 and RIL 25, HA 11-3 and RIL 25 and FPB 21 and RIL 25 were polymorphic to most number of SSR markers. Between these three pairs,relatively large number of alleles was detected. The three pairs of genotypes which were contrasting for both phenotypic traits as well as a set of SSR markers are suitable for use as putative parents for developing mapping population in dolichos bean.