Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 1, 2016; Page No.(257-261)

DETECTION OF GELE AND FACTORS AFFECTING GELATINASE ACTIVITY OF ENTEROCOCCUS FAECALIS ISOLATES

M. PRASANTHA, MEERA RAJUB, S. DIVAKARB, S. ABIRAMIB, H. HAJARAHB, K. UMA HARINIB, S. SENTHILNATHANB AND BENEDICT PAUL C.B

Abstract

Virulence factors of E. faecalis have been found to be involved in the pathogenesis of dental diseases. In this study, prevalence of gelatinase and the effect of factors such as glucose concentration and pH have been investigated. 62% of the isolates (n=26) were positive for gelE in 42 E.faecalis isolates which were amplified in PCR. Among the gelE positive isolates, 65% (n=17) of the isolates were from Dental Caries. The results showed increasing activity of gelatinase as the concentration of glucose increased upto 1%. We also observed that the gelatinase retained its activity between pH 6 and 8.

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