Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 1, 2016; Page No.(31-34)

ISOLATION AND SCREENING OF ANTIMICROBIAL-PRODUCING ACTINOMYCETES FROM MARINE SEDIMENT OF GALESONG COAST, INDONESIA

RAHMITA BURHAMZAH, M. NATSIR DJIDE, HERLINA RANTE AND ELMI N. ZAINUDDIN

Abstract

Isolation and screening of antimicrobial-producing marine actinomycetes has been conducted on isolates taken from marine sediment samples which were collected from various depths of Galesong Coast. Isolation was carried out by using heat shock treatment (heat at 50o C for 10 minutes) and isolates were grown in Starch Nitrate Agar medium added with Nistatin as antifungal. The results obtained 2 isolates of actinomycetes. The screening revealed that isolate GLS-01 has antibacterial activity against Staphylococcus aureus and Escherichia coli which was then fermented in Starch Nitrate Broth for 11 days. In the test of antimicrobial activity of ethyl acetate extract of fermented isolate GLS-01 with concentrations of 3%, 1.5%, 0.375%, and 0.1875% against Staphylococcus aureus resulted the diameters of inhibition of 14.5 mm, 12.51 mm, 12.31 mm, and 12.26 mm, respectively, and against Escherichia coli resulted the diameters of inhibition of 10.88 mm, 9.65 mm, 9.03 mm, and 7.88 mm, respectively. Based on macroscopic and microscopic features, isolate GLS-01 was suspected to be Streptomyces sp.

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