Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 4, 2015; Page No.(1033-1035)

EFFECT OF ARGAN OIL-HYDROGEN PEROXIDE MIXTURE ON MYCOBACTERIUM TUBERCULOSIS IN VITRO

ANWAR K. AL-SAFFAR AND HUSSEIN O.M. AL-DAHMOSHI

Abstract

Objective: Tuberculosis (TB) is the threatful infectious disease that dwells in about one third of worlds. Among Middle East, Iraq occupy the fifth order country endemic with TB. Methodology: Six mixtures of Argan oil:1.5%H2O2 were prepared at different ratio:1=0.75:9.25, 2=1:9, 3=1.5:8.5, 4=2:8, 5=2.5:7.5, 6=3:7 and mixed with Lowenstein-Jensen (L-J) medium. Seventeen Mycobacterium tuberculosis isolates got from the consultants clinic of respiratory and thoracic diseases in Hilla city-Iraq. All isolates cultured on LJ medium containing the mixture of Argan oil: 1.5% H2O2 in different ratio and control tube (LJ without mixture) also used. The growth monitored per a day and recorded at different interval and the inhibition (no growth) were recorded. Results: The effect of Argan oil: 1.5%H2O2 mixture on the Mycobacterium tuberculosis were investigated over three incubation intervals (14th -20th, 21st-28th and after 29th day). The results revealed the high Argan concentration mixture 2.5:7.5 and 3:7 give excellent inhibitory effect on Mycobacterium tuberculosis during full periods of incubation with inhibition percentage (64.71%-82.35%). Conclusion: The current study aims to investigate the effects of argan oil on Mycobacterium tuberculosis and conclude the anti-mycobacterium activity of argan oil as a safe medication for prophylaxis and treatment of TB.

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