Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 4, 2015; Page No.(1005-1014)

BIOCHEMICAL CHARACTERIZATION AND ANTI–OXIDANT ACTIVITY OF PURIFIED L–GLUTAMINASE AND PEG–PHB– GLUTAMINASE NANOCONJUGATES

SURESHBABU RAM KUMAR PANDIAN, VENKATARAMAN DEEPAK, HARIHARAN NELLAIAH AND KRISHNAN SUNDAR

Abstract

Anti–oxidants are essential nutrients that help cancer patients to succeed the cancer treatment effectively with reduced side effects. In the present study an attempt has been made to determine the anti–oxidant effect of PEG–PHB Glutaminase nanoparticles under in vitro conditions. Polyhydroxybutyrate (PHB) was synthesized by a marine bacteria Alcaligenes faecalis and it was converted to PHB nanoparticles by solvent–displacement method. Structure of the bacteria, inclusion bodies in cytoplasm, physical and chemical nature of PHB were studied by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Differential Scanning Colorimetry (DSC) and Fourier Transform Infrared Spectroscopy (FT–IR), respectively. L–Glutaminase enzyme was pegylated, subsequently, immobilized on PHB nanoparticles. Immobilized nanoparticles were characterized by SEM as round shape and around 100 nm in size. The activity and stability of purified and immobilized enzymes were studied biochemically and then, the functional changes of nanoparticle conjugates were studied by FT–IR. The efficacy of free and immobilized enzyme on reducing iron was examined by DPPH, FRAP and power reducing measurement assays. Based on the experimental findings, it can be concluded that the free and immobilized L– Glutaminase has the power of reducing irons as a potential anti–oxidant.

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