Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 4, 2015; Page No.(921-929)

ANTIBACTERIAL PROPERTIES OF CRUDE LYOPHILIZED EXTRACTS OF BACILLUS SUBTILIS AGAINST COAGULASE NEGATIVE STAPHYLOCOCCI CNS

MARWAN YOUSIF HUSSAIN AND ADNAN ALI-NIZAM

Abstract

The aim of this research was to investigate antimicrobials from crude lyophilized extract and used against CNS strains were isolated from clinical and environmental samples. Six strains were isolated from soil and wastewater. The strains were identified by biochemical and 16S DNA methods, another hand, CNS strains were isolated from patients, healthy skin, soil, and wastewater and identified by biochemical methods. The crude antibacterial production was extracted as 3 d. incubation shaker at 37 oC/150 rev min-1, then centrifugation 12000 rev min-1 for 15 min. at 4oC, and filtrate throw 0.45 μm, next freeze and lyophilized. The crude extracts were run on Diamonsil C18 column (5 μm, 250 × 4.6 mm) in HPLC system to separate the antibiotics, also run on FTIR spectrum. Some crude lyophilized extracts which extracted from B. subtilis gave high activity against some strains of CNS and others are not. Two kinds of antibiotics (Bacitracin A and Polymyxin B) were isolated from B. subtilis and identified to the peptide family. Bacitracin A could function as antibacterial against a wide spectrum of CNS pathogens.

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