Ecology, Environment and Conservation Paper

Vol.16, Issue 2, 2010; Page No.(319-324)

EVALUATION OF CONSTITUTIVE AND INDUCED BIOCHEMICAL DEFENSE MECHANISMS IN OKRA [ABELMOSCHUS ESCULENTUS (L.) MOENCH] GENOTYPES AGAINST YELLOW VEIN MOSAIC VIRUS

Amandeep Kaur and Neelima Arora

Abstract

The present study was carried out to evaluate constitutive and induced biochemical defense mechanism in seven okra genotypes, Hissar-108-2, NDO 10, POS-1, POS-26, Punjab-8, Pusa Sawani and Pusa Makhmali for resistance against yellow vein mosaic virus. Resistant genotypes recorded higher levels of non-enzymatic antioxidants viz total phenols, flavonoids and ascorbic acid at both pre-infectional and disease initiation stages. Level of these antioxidants increased in healthy as well as diseased leaves at disease initiation stage. However, the increase was higher in healthy plants as compared to the diseased plants. Elevated activities of peroxidase, polyphenol oxidase, phenylalanine ammonia lyase and superoxide dismutase was observed in resistant genotypes compared to the susceptible genotypes. At stage II, the enzyme activities increased approximately two folds in healthy plants whereas the increase was comparatively lesser in diseased plants. However, superoxide dismutase activity was higher in diseased plants as compared to healthy plants.

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