Ecology, Environment and Conservation Paper

Vol. 21 Dec. 2015 Suppl. Issue; Page No.(59-63)

MOLECULAR DIVERSITY ANALYSIS OF WHITEFLY (BEMISIA TABACI) COLLECTED FROM DIFFERENT REGIONS OF EAST MADHYA PRADESH, INDIA

Sumit Kale, Sharad Tiwari, Niraj Tripathi and Keerti Tantwai

Abstract

This study aimed to employ DNA based method to clarify the existing of biotype among whitefly populations in East Madhya Pradesh, India. The genomic DNA of whitefly collected from different geographical regions of East Madhya Pradesh. The mtCOI primer was used for identification of B biotype. Specific mtCOI primer CI-J-2195 and L2-N-3014 showed the presence of 880bp bands which confirmed the presence of B biotype in different geographical regions of East Madhya Pradesh. All identified B biotypes were used for molecular diversity analysis. The DNA samples were amplified with 5 RAPD primers in thermal cycler. Four decamer primers amplified 8 RAPD marker loci. All 8 alleles scored by RAPD markers found to be monomorphic. Average number of bands per primer was 2.00. No variation was found among whitefly samples collectedfrom different geographical regions of East Madhya Pradesh.

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