Ecology, Environment and Conservation Paper

Vol. 21 Dec. 2015 Suppl. Issue; Page No.(31-34)

MOLECULAR MARKER ANALYSIS FOR TOLCV RESISTANCE IN F2 POPULATION OF TOMATO (SOLANUM LYCOPERSICUM (MILL.) WETTSD)

V. M. Choudki and O. Sridevi

Abstract

An investigation on molecular markers for ToLCV resistance in mapping populations of tomato was undertaken to detect trait related markers linked to ToLCV resistance. CLN2768A (Resistant) Pusa Ruby (Susceptible) was used as parents. F1 plants of the cross were found to be resistant. Genomic DNA of parents, F1 and F2 population of the cross characterized using 66 selected primers from SGN network. Though amplification was obtained with all the 66 primers, polymorphism was produced by only 6 primers. Primer TG0302 gave a unique band of size 900 bp and 800 bp in CLN2768A and Pusa Ruby and in resistant and susceptible bulks from F2 population. When the whole F2 population of was analyzed individually with the primer TG0302, DNA of all the resistant plants showed 900 bp band. In the case of susceptible plants, a segregation of polymorphic band was observed. All this suggests that the gene for resistance to ToLCV in Pusa Ruby is dominant in nature and the polymorphic band produced by the primer TG0302 could be related to ToLCV resistance in tomato.

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