ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF DIDYMOCARPUS TOMENTOSA WIGHT: AN ENDEMIC PLANT OF PENINSULAR INDIAJ. PRAMEELA, H RAMAKRISHNAIAH, V KRISHNA AND A P DEEPALAKSHMIAbstract Didymocarpus tomentosa is a medicinal plant used traditionally to treat fever and skin allergy. The aim of the present study was to evaluate the in vitro antimicrobial activity of leaf and root extracts of D. tomentosa against Bacillus subtilis, Klebsiella pneumonia, Salmonella typhi and Candida albicans using agar welldiffusion method. Minimum inhibitory concentration was also determined. The cytotoxic activity was assessed by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2 H-tetrazolium bromide assay. The extracts exhibited a significant antimicrobial activity against all the tested microorganisms, with minimum inhibitory concentration ranging from 125 to 1000 μg/mL. The leaf extract exhibited a superior cytotoxic activity on Human cervical carcinoma cell-line with IC50 value of 87.6 μg /mL than root extract which showed 676.2 μg/ mL. The results of the present study indicate that the methanolic extracts of Didymocarpus tomentosa demonstrated highly significant antimicrobial and moderate cytotoxic activities. The present study is the first report on the antimicrobial and cytotoxic activities of D. tomentosa extracts.
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