A COMPARATIVE STUDY ON BIO-POLYMER (ε-POLY-L-LYSINE) PRODUCTION BY USING FOREST SOIL MICROBIAL ISOLATES AND STREPTOMYCES ALBULUS (MTCC 1137)ARUN A, K. MOHAN RASU, G.H. DINESH, R. SATHEESH MURUGAN, G. SIVA PRAKASH1 AND V. ANANTHI
Lack of the essential amino acid L-Lysine in cerals has triggered studies on L-Lysine production by both chemical and microbial methods. In the recent years a considerable interest in biopolymers exist as they have huge potential in variety of application. Poly ε - L-lysine (ε - PL) is a basic homo polymer that consist of 20 to 30 residues of L-lysine with an ε-amino and alpha carboxyl group linkage. ε-PL has a range of industrial application such as food preservative, dietry agent, emulsifying agent, biodegradable fibers, anticancer agent, drug carriers and antimicrobioal activity against a wide spectrum of microbes. The potential ε-PL producing microbes were collected from Sathuragiri Hills, Kodai Hills, Thandikudi Hills of Western ghats. The isolated ε-PL producers were screened for their ability to produce ε-PL in shake flask culture method. The potential ε-PL producers such as MS-1, MS-2 and MS-3 were selected and used for further studies. To compare the level of ε-PL produced by the selected organisms with a control organism such as Streptomyces albulus was obtained from MTTC Chandigarh, India and was used in the study. Pure form of ε-PL produced by MTTC 1137, MS1, MS2 and MS3 were extracted and the quality was confirmed by FTIR spectrum. Batch type of fermenter (3 litre fermentor) was used in the study using the best isolate such as Bacillus cereus (MS-1). The fermenter was used in the optimized condition and Bacillus cereus (MS- 1) was able to produce a maximum level of 34.1g/L ε-PL at 60th hour.
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