APOPTOSIS OF HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) MEDIATED BY RECOMBINANT WOLBACHIA SURFACE PROTEIN (RWSP) FROM BRUGIA MALAYIHARIPRIYA KALYANARAMAN, KIRTHIKA SREENIVAS, KAMALAKANNAN VIJAYAN AND RANGARAJAN BADRI NARAYANANAbstract The inflammatory response and thereby the pathogenesis of filarial infections are ascribed to the presence of the intracellular bacterium of filarial nematode, Wolbachia. Wolbachia associated antigen, i.e Wolbachia Surface Protein (WSP) is known to contribute to immune modulation of human host, but its role in the presence of a parasite antigen (Brugia malayi pepsin inhibitor, Bm33) has not been studied. Hence, recombinant WSP (rWSP) was produced and its immunological role in apoptosis induction was investigated using human PBMCs. Acridine orange/Ethidium bromide staining showed that rWSP induced apoptosis in the presence and absence of recombinant Bm33 (rBm33). This was further substantiated with enhanced gene expression of pro-apoptotic marker, Bax and elevated levels of pro-inflammatory cytokines TNF-α and IL- 6 compared to rBm33 and control. Thus it is evident from the above findings that rWSP induces apoptosis irrespective of the presence of the parasite antigen in human PBMCs.
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