Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 3, 2015; Page No.(611-614)

MICRO PROPAGATION STUDIES IN PAPAYA (CARICA PAPAYA L.) CV. PUSA DWARF

RAJESH SAMANTARAY, S.P. SINGH, PRIYANKA SINGH AND RAJESH SHALITRA

Abstract

An experiment was carried out for micro propagation studies in papaya (Carica papaya L.) cv. Pusa Dwarf in the Department of Horticulture, Institute of Agricultural Sciences, BHU, India during 2011-2013. Immature zygotic embryos were used as explants for multiplication in order to minimize the somaclonal variation. The plant growth regulator 2, 4-D (4 mg/L) was adjudged excellent for callusing. BAP (2 mg/L) + NAA (0.2 mg/L) resulted in best treatment for shoot proliferation considering sprouting percentage, number of shoots and shoot length. IBA (2 mg/L) + NAA (0.2 mg/L) proved to be best for days to root initiation, rooting percentage, root length and number of roots. IBA (2mg/L) + activated charcoal (200 mg/ L) was found superior for days to root initiation, number of roots and root length, while ascorbic acid (50 mg/L) resulted in 100% browning free culture.

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