SUGAR CONSUMPTION IN MONO AND CO-CULTURE SACCHAROMYCES CEREVISIAE AND OTHERS SELECTED MICROORGANISM FOR BIOETHANOL PRODUCTION FROM STREAM RICE HUSK MEDIUMT. SOPANDI AND A. WARDAH
This study sought to explore potential of mono and co-culture fermentation of S. cerevisiae with C. tropicalis, Z. mobilis and P. resticulosum for bioethanol production from stream rice husk medium. Batch fermentation was conducted in a waste rice husk hydrolysate basal medium with 4 g/L urea, 3 g/L NaNO3, 3 g/L NH4NO3, 1 g/L KH3PO4 and 0.7 g/L MgSO47H2O supplemented and 3 days (30oC, 60-70% relative humidity, dark, 150 rpm agitation) incubation. Maximum ethanol production (2.125±0,259 %/L) was gained from co-culture S.cerevisiae, C. tropicalis with efficiency fermentation 89.25±10.95%. Co-culture S.cerevisiae, C. tropicalis have ability to generate and convert fermentable sugars from a waste stream rice husk to ethanol. Co-culture S. cerevisiae, C. tropicalis demonstrated high potential and such application provides a process for agricultural waste stream resource reuse for production of ethanol in increasing demand.
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