Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 3, 2015; Page No.(577-586)

SUGAR CONSUMPTION IN MONO AND CO-CULTURE SACCHAROMYCES CEREVISIAE AND OTHERS SELECTED MICROORGANISM FOR BIOETHANOL PRODUCTION FROM STREAM RICE HUSK MEDIUM

T. SOPANDI AND A. WARDAH

Abstract

This study sought to explore potential of mono and co-culture fermentation of S. cerevisiae with C. tropicalis, Z. mobilis and P. resticulosum for bioethanol production from stream rice husk medium. Batch fermentation was conducted in a waste rice husk hydrolysate basal medium with 4 g/L urea, 3 g/L NaNO3, 3 g/L NH4NO3, 1 g/L KH3PO4 and 0.7 g/L MgSO4•7H2O supplemented and 3 days (30oC, 60-70% relative humidity, dark, 150 rpm agitation) incubation. Maximum ethanol production (2.125±0,259 %/L) was gained from co-culture S.cerevisiae, C. tropicalis with efficiency fermentation 89.25±10.95%. Co-culture S.cerevisiae, C. tropicalis have ability to generate and convert fermentable sugars from a waste stream rice husk to ethanol. Co-culture S. cerevisiae, C. tropicalis demonstrated high potential and such application provides a process for agricultural waste stream resource reuse for production of ethanol in increasing demand.

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