Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol. 17 Special Issue 2015; Page No.(41-44)

ASSESSMENT OF GENETIC DIVERSITY IN SALACIA CHINENSIS USING RAPD ANALYSIS

RESHMA B. NAMBIAR, S. PERIYAR SELVAM, AND IDA IDAYU MUHAMAD

Abstract

Successful management and conservation of medicinal plants depend on precise evaluation of genetic diversity which helps in addressing questions regarding genetic relationship among individuals as well as genetic variation. Random amplified polymorphic DNA (RAPD) markers are useful tool to provide a convenient and rapid assessment of the genetic differences between genotypes. In the present study genetic variations of Salacia chinensis were determined by RAPD analysis. The plant material was collected from different region such as Kerala and Puducherry and analysed for genetic variation through two RAPD primers such as OPC 13 and OPC 14. The OPC 13 primer produced brighter bands at 2000, 4000 and 600 bp for the leaf samples of both Kerala and Pondicherry, whereas a band was only present at 550 bp in Puducherry sample. The OPC 14 primer showed bright bands at 3000 bp and 1250 bp in Puducherry samples, however both the samples showed bands at 2000, 1500 and 450 bp. The leaf samples of Salacia chinensis from Kerala and Puducherry regions almost showed similar pattern with OPC 13 primer and OPC 14 primer although only slight genetic variation was found with both the primer. However further study is needed for confirmation of genetic variation.

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