A COMPARATIVE ANALYSIS ON MICROBIAL PRODUCTION OF PRIMARY ALCOHOLS USING PRETREATED GLYCEROLCHOZHAVENDHAN SIVASANKARAN PRAVEEN KUMAR RAMANUJAM, SIVARATHNAKUMAR SHANMUGAM, KIRUBALINI GOVINDARAJ, BHARATHIRAJA BALASUBRAMANIANAND JAYAKUMAR MANIAbstract Crude glycerol is effectively utilized as substrate for the production of value added bio products such as acetic acid, citric acid, DHA, ethanol, etc. It has several advantages over commercial substrates which includes low cost nutrients, as a good carbon and energy source for the microorganisms to grow with high product conversion rate. In this research ethanol a primary alcohol, an alternate biofuel was produced by various bacterial sp. and the yield of ethanol were compared. The crude glycerol is pretreated and neutralised with H3PO4, further centrifuged to remove impurities present in it, which helps to increase the concentration of glycerol and yield. The production of ethanol from purified glycerol was compared by maintaining the same condition with various organisms .The product obtained from purified glycerol fermentation was confirmed as ethanol by simple qualitative test and further by GC-MS. When compared with other organisms, concentration of ethanol obtained from Zymomonas mobilis, on purified glycerol using phosphoric acid yields 5.1 %.
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